HPLC Purity & Identity Analysis

High-Performance Liquid Chromatography (HPLC) Analytical Testing

Nuvio Diagnostic

Nuvio Diagnostic provides in-house HPLC analytical testing using Agilent 1100 Series instrumentation and qualified reference standards. Our services support compound identification, purity determination, concentration verification, and batch consistency evaluation for research and quality verification purposes only.

All analyses are performed under controlled laboratory conditions using validated or fit-for-purpose methods, including USP-aligned validation where applicable.

HPLC Testing Services

Quantitative & Qualitative Analysis

• Compound identification

• Purity determination

• Concentration verification

• Batch / lot consistency evaluation

Quantitative Assay (With Reference Standards)
Compound concentration is determined using external calibration curves generated from qualified reference standards.

Peptide Purity & Quantity Testing

Nuvio Diagnostic offers peptide purity and quantity testing for a wide range of research peptides, including:

Semaglutide • Tirzepatide • Retatrutide • PT-141 • Melanotan II • BPC-157 • TB-500 • TB-4 • GHRP-2 • GHRP-6 • SS-31 • MOTS-c • Thymosin Alpha-1 • Kisspeptin • Tesamorelin • HGH Fragment 176-191 • Epithalon • GHK-Cu • AOD-9604 • Selank • Mazdutide • TB-500 Fragment 17–23 • SNAP-8 • Thymalin • CJC-1295 • Oxytocin

If your peptide is not listed, please contact us. New analytical methods are continually added.

How Testing Works

• Sample Preparation: Samples are dissolved in ultra-pure water and prepared for analysis

• Chromatographic Separation: RP-HPLC using peptide-specific C18 columns

• Detection: UV detection at 214–220 nm (peptide bond absorbance)

• Quantification: Comparison of sample peak areas to authenticated external standards

• Validation: Methods aligned with USP <1225>, where applicable

Additional Capabilities

• Relative purity analysis

• Identity confirmation via retention time matching

• Impurity & degradation profiling

• Batch / lot consistency testing

• Stability screening

• Method transfer, replication, and custom analytical requests

Instrumentation

• Agilent 1100 Series HPLC

• UV-Vis Detection (DAD / VWD)

• External standard calibration (method-dependent)

Turnaround Time

3–5 business days from sample receipt for standard purity and quantity panels.

Sample Submission

Samples may be mailed in with a completed submission form or dropped off in person Monday–Friday, 8:30 AM – 5:30 PM (no appointment required).

Important Notes

All services are provided for research and analytical purposes only.
No clinical, diagnostic, or medical testing is performed.